ranges of dna concentration in plant

Accurate estimation of homologue-specific DNA concentration

Cite this article. Carter, S., Meyerson, M. & Getz, G. Accurate estimation of homologue-specific DNA concentration-ratios in cancer samples allows long-range haplotyping.

Guidelines for Sanger sequencing and molecular assay …

The optimal values for 260 nm/230 nm ratio calculations are 2.0–2.2. 25 The guidance on DNA concentration values provided herein is based on a single amplicon in the sample; samples containing multiple amplicons (e.g., from multiplex PCR reactions) add to the total weight of the measured DNA and will provide false assumptions when …

A Comprehensive High-Quality DNA and RNA Extraction …

The method presented here yielded a high quantity and concentration of DNA from 1 g of plant tissue for other plants, including capsicum ... Recommendations established here could prove adaptable to extract high-quality nucleic acids from tissues from a range of difficult plant species. Acknowledgments. The authors wish to …

Nucleic Acid Quantitation

Quantification and analysis of nucleic acids. Chang-Hui Shen, in Diagnostic Molecular Biology (Second Edition), 2023. Real-time PCR method. The qPCR technique is a powerful nucleic acid quantitation method. This is a good choice for qualitative as well as quantitative analysis of DNA because of its high sensitivity and specificity for typical …

DNA Source Selection for Downstream Applications Based on DNA …

DNA concentration is generally calculated using Lambert–Beer law from spectrophotometric analysis of the absorption at 260 nm ... The measurement of DNA concentration at a lower range can be strongly affected by light scattering on dust particles present in the sample. This method for measuring concentration is relatively …

General Recommendations for Protocol DNA …

Recommended Agarose Gels for Electrophoretic Separation of DNA Fragments. Agarose gel, % Range of effective separation, bp Approximate positions of tracking dyes, bp* Bromophenol blue Xylene cyanol FF TBE buffer TAE buffer TBE buffer TAE buffer 0.5 2000-50000 750 1150 13000 16700

Brian Matlock, Thermo Fisher Scientific, Wilmington, MA, …

in the range of 2.0–2.2. Residual chemical contamination from nucleic acids extraction procedures may result in an overestimation of the nucleic acid concentration and/or negatively influence downstream analysis. Figure 1 shows an example spectra for four common extraction reagents which, if not properly cleaned up, will affect sample purity.

DNA concentration and quality in plant extractions. (A) Concentration …

DNA concentration and quality in plant extractions. (A) Concentration (ng/ μ L) ± 1 SE as detected by the Epoch Spectrophotometer System determined for seven replicates. ... The DNA size range ...

Testing protocols to optimize DNA extraction from tough …

(A) Median and quartile concentration range of DNA yielded from tissue stored on silica since 2012, 2019, or 2022. Significance is denoted by a blue asterisk. (B, C) Median and quartile range of DNA purity from tissue stored for different durations, represented by A 260 /A 280 (B) and A 260 /A 230 (C) absorbance ratios.

Extraction of high-quality genomic DNA from different plant …

The present work describes an inexpensive CTAB-based method with modifications for the extraction of high-quality genomic DNA from 19 different plant …

Plants | Free Full-Text | A Comprehensive High-Quality DNA …

The method presented here yielded a high quantity and concentration of DNA from 1 g of plant tissue for other plants, including capsicum (Supplementary Material Figure S1 ... "A Comprehensive High-Quality DNA and RNA Extraction Protocol for a Range of Cultivars and Tissue Types of the Woody Crop Avocado" Plants 11, no. 3: …

Plant Tissue Phosphorus Sufficiency Ranges

While soil testing is performed to predict the nutrient availability in soils, plant tissue analysis provides information on the nutrients taken up by the plants. The phosphorus concentration in plant tissue might be in deficiency range, sufficiency range, or excess range.

How to determine the concentration and purity of a DNA …

Reliable measurement of DNA concentration and purity is important for many applications in molecular biology where accurate determination of DNA concentration is critical. ... If the ratio is out of the general acceptable range, it may indicate the presence of contaminants which absorb at 230 nm. ... Carbohydrates are a …

DNA analysis using analytical gels

Agarose concentration (% w/v) DNA fragment range (kb) 0.3* 5–60: 0.5: 1–30: 0.7: 0.8–12: 1.0: 0.5–10: 1.2: 0.4–7: 1.5: 0.2–3: ... This will depend on the size of DNA being analyzed, the concentration of agarose in the gel, and the separation required. ... Plant tissue; Fungal material; DNA cleanup. Isopropanol precipitation of DNA.

Seasonal change in environmental DNA concentration of a …

Abstract Environmental DNA (eDNA) methods are increasingly used to detect aquatic organisms. To optimize survey efficiency in natural environments, it is necessary to understand the seasonal change in eDNA concentrations of target species and identify the season and environmental conditions in which eDNA concentrations are highest. …

A universal protocol for high-quality DNA and RNA isolation …

Next-generation sequencing demands high-quality nucleic acid, yet isolating DNA and RNA is often challenging, particularly from plant tissues. Despite advances in developing various kits and reagents, these products are tailored to isolation of nucleic acid from model plant tissues. Here we introduce a universal lysis buffer to separate nucleic …

Improving DNA quality extracted from fecal samples—a

DNA was successfully recovered from all of the samples. The E. hemionus was the species yielded a higher DNA amount, with an average of 71.12 ng/μl, ranging from 7.39 to 179 ng/μl. The E. africanus samples produced DNA concentrations between 9.90 and 257 ng/μl, with an average of 40.16 ng/μl.E. kiang produced an average of 24.97 …

A high throughput DNA extraction method with high yield …

The method combines a traditional CTAB DNA extraction and modified DNA clean-up procedure using silica-coated magnetic beads into a new platform that reduced …

Comparison of three common DNA concentration measurement methods

The resulting DNA concentration was significantly lower for some of the protocols (Table 1), indicating that the original eluent from those protocols contained large amounts of RNA contamination.

Quantifying DNA? Here are Five DNA Quantification …

An optimized protocol for stepwise optimization of real …

Transcript abundance of the eight genes was also measured at different inflorescence developmental stages of T. ravennae (i.e., 40, 80, 120, 160, and 200 cm in length). The average Ct values of ...

Guidelines for PCR Optimization with Taq DNA Polymerase

Taq DNA Polymerase Concentration. Use 0.5–2.0 units per 50 µl reaction, ideally 1.25 units; Starting Reactions. Assemble all reaction components on ice; ... Typical annealing temperatures are 5°C below the lowest primer's T m and often fall in the range of 50-60°C; Test higher annealing temperatures if spurious amplification products are ...

The Nanodrop Spectrophotometer: Quantification Made …

A NanoDrop spectrophotometer is a common lab instrument that can measure the concentration of DNA, RNA, and protein in a 2-µL drop on a pedestal. ... The NanoDrop's Linear Dynamic Range. The linear dynamic range of the NanoDrop spectrophotometer varies depending on the model (see Table 2). Table 2.

DNA Quantification

Calculate your DNA sample concentration and purity using Addgene's DNA Quantification Protocol.

qPCR Quantification Protocol Guide

quantifying DNA based on PCR. qPCR tracks target concentration as a function of PCR cycle number in order to derive a quantitative estimate of the initial template concentration in a sample. As with conventional PCR, it uses a polymerase, dNTPs, and two primers designed to match sequences within a template.

DNA extract characterization process for microbial detection …

The DNA concentration measurements made using the Nanodrop were ... steps may have resulted in loss of genomic DNA. A range of different size beads are used in the bead beating step of precipB which may aide in lysis of different cell types resulting in ... based analysis of fungal and bacterial DNA. Plant Mol Biol Rep. 2004; 22:71–81 ...

Rapid high throughput template preparation (rHTTP) method…

Background Conventional plant DNA isolation methods are complex, time consuming and require technical expertise. These limitations were overcome using the DNA isolation kits which, however significantly add to the research costs. Hence the present study was aimed to develop a high throughput, rapid and inexpensive method of PCR …

Isolation and Purification of DNA from Complicated Biological Samples

DNA isolation from filters was performed using two protocols to compare their efficiency (Tables S2 and S3). The first protocol is based on the use of 1% CTAB solution in the presence of 1 M NaCl ...

Agarose Gel Electrophoresis for the Separation of DNA …

Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb 1.Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose) subunits 2.During gelation, agarose polymers associate non-covalently and …

DNA quantification methods | INTEGRA

Accurate DNA quantification is key for many molecular biology workflows. Without precise knowledge of your sample's concentration, yield and purity, the success of downstream applications can be put at risk. This article describes the different methods available for quantifying DNA, highlighting their strengths, weaknesses and the essential …